Scientific Program

Conference Series Ltd invites all the participants across the globe to attend 19th World Conference on Environmental Toxicology and Pharmacology Tokyo, Japan.

Day 2 :

Conference Series EnviTox Summit 2019 International Conference Keynote Speaker Lidia Zapór photo
Biography:

Lidia Zapór works in the Department of Chemical, Aerosol and Biological Hazards in the Central Institute for Labour Protection – National Research Institute (CIOP-PIB). She is a head of Laboratory of Toxicology. The main area of her professional interest are problems of human in the working environment and the toxicity of chemical substances as well as methods of estimating the toxicity of substances in vitro. She was also engaged in preparation of documentation of maximum allowable levels of occupational exposure and characteristics of hazardous substances in the work of the Interdepartmental Committee mandated with updating and verification of the Threshold Limit Values (TLVs) for agents harmful to human health.

 

Abstract:

Statement of the Problem: Nanostructured molybdenum trioxide (MoO3-NPs) is promising material in many applications: in coatings, plastics, textiles, pigments, lubricants, ceramics and glass production, as antimicrobial agents, and for the detection of dopamine in pharmaceutical and clinical preparations. In recent years, it was noted that in the case of nanomaterials should carefully evaluate the risks of their use, as they may pose a health risk. The objective of this study was to assess the cytotoxic activity of MoO3-NPs in human pulmonary cells.

Methodology: The cytotoxicity of MoO3-NPs was assessed on the alveolar carcinoma epithelial cells (A549) and normal bronchial epithelium cells (BEAS-2B) after short, and long-term time of exposure. Cytotoxicity studies included the effect of MoO3-NPs on cell viability, cell membrane integrity (NRU assay), mitochondrial metabolic activity (MTT assay) and the ability of the cells to proliferation (Clonogenic assay).

Findings: MoO3-NPs induced a dose- and time-related negative effect on the viability of both kids of the cells in the cytotoxic doses range 50 - 300 µg/ml, depending on cytotoxicity endpoint. In long-term exposure (7 day), MoO3-NPs at concentrations about 100 µg/ml impaired proliferation, implying their potential chronic toxicity. A549 cells were less sensitive than BEAS-2B one, to all measurement parameters.

Conclusion & Significance: The sensitivity of BEAS-2B cells to MoO3-NPs is of particular concern. These cells form a defense line of the body against the penetration of particles into lungs. Inhibition of the ability of BEAS-2B cells to proliferate under the influence of MoO3-NPs may be an unfavourable phenomenon for predicting their long-term effects of exposure.

Funding. This paper has been based on the results of a research task II.N11A. carried out within the scope of the fourth stage of the National Programme Improvement of safety and working conditions partly supported in 2017–2019 — within the scope of research and development — by the Ministry of Science and Higher Education/National Centre for Research and Development. The CIOP-PIB is the Programmes main co-ordinator.

  • Toxicology
Location: Radisson Hotel Narita
Speaker
Biography:

Shikha Singh is currently working as a Doctoral Fellow at University of Allahabad, India. She has completed her Masters of Science with cytogenetic specialization from University of Allahabad, India. She has presented ten oral posters in different international/national conferences organized in India. She has been awarded with Best Oral Presentation award for her work in Indian Science Congress Association. She also assists as resource person in various academic courses.

 

Abstract:

The application of chemical pesticides to agricultural land very often contaminate aquatic habitat which in turn causes detrimental effects to the aquatic biota particularly to the economically important non-target organisms like fish. The aim of the present study was to explore the impact of pesticides (triazophos, deltamethrin and their combination) on oxidative stress level in fish, Channa punctatus. The fishes were procured from local animal supplier of Allahabad, India and acclimatized in laboratory condition. The fishes were exposed to different concentrations of pesticides for 96 hours. The LC50 (Lethal Concentration) value for triazophos, deltamethrin and their combination were found to be 0.069 mg/l, 7.17 µg/l and 0.032 mg/l respectively. For the sub-lethal study, fishes were exposed to 5% and 10% of LC50 of pesticides for 96 hours. During the exposure period, the behavioral changes (i.e. opercular movement, surfacing) were observed. Immediately after exposure, the animals were sacrificed and blood and the key organs (brain, liver, kidney, gills and muscles) were collected for biochemical/stress enzymes assay and for apoptotic studies. In the blood parameters, TLC and DLC showed significant change in counts as compared to control with increased rate of apoptosis. In the stress related enzyme activity such as SOD, CAT, GST and levels of GSH and LPO (Lipid Peroxidation) significant changes were recorded with increase in concentration of pesticides. From our study conclude that, the dose dependent exposure of pesticides may impose detrimental threat to the fish population.

 

Speaker
Biography:

Rishikesh Kumar Tiwari (Doctoral Fellow): Presently working as a Doctoral fellow with Prof. Ravi S. Pandey, Biochemistry Laboratory, Department of Zoology, University of Allahabad, Mr. Rishikesh K. Tiwari has completed his M.Sc. with cytogenetics specialization from University of Allahabad, India. He has presented ten oral/poster in different International/National Conferences organized in India besides ten research/review articles in National/International journal repute and one book chapter in USA based publishing house. He has attended one International workshop and assisted as resource person in various academic course(s).

 

Abstract:

The increasing applications of pesticides in the agricultural fields have adverse impact on flora and fauna of the soil ecosystem. The role of earthworms in the agricultural practices is well known as they immensely contribute in increasing the quality and fertility of soil. So, it acts as a bioindicator for the ecotoxicological analysis of pesticide induced soil pollution. Therefore, the present study was aimed to explore the impact of chlorpyrifos (an organophosphate; OP), cypermethrin (a pyrethroid) and their combination (chlorpyrifos + cypermethrin) on earthworm, Eudrilus eugeniae.   E. eugeniae were exposed to different concentrations of pesticides for 48 h by paper contact toxicity method.  The LC50 for commercial grade chlorpyrifos, cypermethrin and their combination were determined as 0.165, 0.066 and 0.020 μg/cm2 respectively. To assess the sub-lethal effect of these pesticides, E. eugeniae were exposed to 5% and 10% of LC50 pesticides for 48 h. Alterations in morpho-behavioural patterns such as coiling, clitellar swelling, mucus release, and bleeding followed by fragmentation of body in earthworms were observed following exposure. Acetylcholinesterase (AChE) activity was assayed in different regions of body segment which exhibits significant (p < 0.05) decrease in AChE activity particularly in pre-clitellar region followed by clitellar and post clitellar regions and in comparison, to whole body. The decreased AChE activity with increasing concentration of pesticides indicates the effect at neuronal level which apparent from the behavioural changes. Therefore, from the present findings it can be concluded that long term exposure to these pesticides could lead to severe and irreparable effects on biochemical mechanisms of earthworms.