Hyun-Suk Oh has her expertise in agrichemical. She is working for risk assessment of hazardous chemicals in Korea Ministry of Food and Drug Safety. She major research field is development of endocrine disrupting chemicals screening methods using cell culture system. Furthermore, she is interested relationship between endocrine disrupting chemicals and hormonal disease by low level exposure in human, animal and in vitro model system.
It is expected that consumption of veterinary drugs will increase due to growing agriculture and aquaculture production which have to meet the demands of expanding human population for food. However, the presence of several veterinary drugs residues in food-producing animals could cause potential endocrine disrupting effects on human. Therefore, Economic Co-operation and Development (OECD) has provided a standardized method to search the effect of chemicals on the endocrine system. The aim of this study is to assess the androgen receptor (AR) agonistic/antagonistic effects on 22 veterinary drugs, which are used as anthelminthic for livestock by OECD in vitro stably transfected transcriptional activation assays using chines hamster ovary cell line, AR-EcoScreenTM(OECD TG458). Among the tested chemicals, three veterinary drugs were determined to AR agonist(Albendazole, Mebendazole amine, Thiabendazole) and antagonist(Cymiazol, Praziquantel, Triclabendazol) by OECD TG458. These data provide information about AR agonistic/antagonistic effects of veterinary drugs by OECD in vitro assays. Although these results may not correlate directly with risks to humans, it would be offer the basal scientific information about food, drug and cosmetic ingredients with safety management. This research was supported by grant (17161MFDS115) from the Ministry of Food and Drug Safety Korea.
Dr. rer. nat. Richard Gminski, is a senior scientist at the Institute of Infection Prevention and Hospital Epidemiology, Medical Center, University of Freiburg, Germany. He holds a degree in pharmacy and was rewarded a doctorate in natural sciences by the University of Heidelberg, Germany. He was scientific assistant in various university departments and medical centers in Heidelberg, Mannheim, Trier, Giessen and Berlin. His current research interests are environmental toxicology and particle toxicology, i.e. molecular mechanisms of fine and ultrafine particles (nanoparticles) with respect to mutagenicity and DNA damage in humans. Further interests are nutrition, forensic medicine and urban global health. He develops new high-throughput assays for rapid screening of biological activity or toxicity of environmental toxins and chemical compounds. He has broad experience in mutagenicity, genotoxicity and carcinogenicity testing of environmental chemicals and has published many papers on these topics.
Despite international efforts to limit worker exposure, coal mine dusts continue to impact the health of thousands of miners across Europe. Modern, practicable assessment tools and devices are urgently needed to protect workers, particularly from the fine fraction (PM2.5), which is increasingly implicated in human disease. To predict dust toxicity of different coal mine dusts and mining scenarios, a set of toxicological assays are necessary to identify a successful improvement of risk management targeting mitigation measures. Various studies indicate that the surface area and the potential to form reactive oxidants are highly promising metrics to predict the toxic potency of fine and ultrafine dusts. In the frame of the European ROCD project, two lignite coal mine dusts with different fractions (PM2.5 and PM10) obtained from an active lignite mine in Eastern Europe were investigated for their cytotoxic, oxidant generating capacity and inflammatory potential in the human alveolar epithelial cell line A549. Furthermore, to relate the observed effects to the hydroxyl-radical (OH•)-generating activities of these samples. The approach is based on the aligned electron paramagnetic resonance spectroscopy (EPR) technique with 5.5-dimethyl-1-pyrroline-N-oxide (DMPO) as spin trap and hydrogen peroxide as substrate, and is specifically sensitive to Fenton-type reaction mediated generation of hydroxyl radicals. The results show that the two lignite coal mine dust samples investigated induce cytotoxic effects, produce ROS and release cytokine IL-8 in a concentration-dependent manner, with a similar potency to the two reference substances quartz and coal fly ash (CFA). Moreover, the toxic effects of the two coal mine dusts observed in human lung cells A549 appear to correlate with the hydroxyl-radical-generating capacities of both coal mine dust samples. The two studied coal mine samples and two reference substances with known constituents reveal the intrinsic hydroxyl-radical-generation method to be a sensitive tool for prediction of adverse health effects.